Thrombin is the last enzyme in the clotting cascade. In this paper, we showed that nanopipettes functionalized with aptamers can be used for the detection of thrombin in serum solutions.
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Chitosan functionalised nanopipettes
The polysaccharide chitosan is the structural element in the exoskeleton of crustaceans (crabs, shrimp, etc.) and is known to form complexes with metal ions. In this paper, we show that a nanopipette functionalised with chitosan can reversibly bind metal ions, resulting in a reversible, real-time signal that is concentration dependent.
Nanoprecipitation in a nanopipette
Studying the earliest stages of precipitation at the nanoscale is technically challenging but quite valuable as such phenomena reflect important processes such as crystallization and biomineralization. Using a nanopipette as a nanoreactor, we induced precipitation of an insoluble salt to generate oscillating current blockades.
Functionalized nanopipettes
In this paper, we review the potential of functionalized nanopipettes as a new platform for label-free biosensing. We highlight the potential of this technology with a particular attention to its integration with single cell manipulation platforms.
Mycotoxin detection by nanopipette
Mycotoxins are secondary metabolites produced by fungi that are capable of causing disease and death in humans and animals. In this paper, we showed that antibody functionalized nanopipettes can detect HT-2 toxin with a detection limit of 100 fg/ml and compared the results with conventional sandwich assay techniques.
Solvent free Diamond functionalization
An ionic liquid is a salt in the liquid state. Ionic liquid have been widely used as clean solvents and catalysts for “green chemistry”. In this paper, we report the “green” chemical functionalization of diamond electrodes with diazonium salts dissolved into ionic liquids.
Diazonium-functionalized Carbon
Diazonium salts have been traditionally employed to produce dyed fabric. In this paper, we coupled Raman spectroscopy with electrochemistry to study the reduction of diazonium molecules covalenty attached to the surface of glassy carbon.
Electrochemical properties of Diamond
Diamond exhibits a number of outstanding properties of technological interest such as extreme hardness, chemical inertness, and high resistance to corrosion. In this paper, we investigated the influence of functional groups on the surface of diamond on its electrochemical properties.
Photografting of DNA on Diamond
Benzophenone is a very efficient photophore that can cross-link biomolecules under mild UV irradiation. In this paper, we describe the covalent grafting and patterning of oligonucleotides onto benzophenone-modified boron doped diamond electrodes.
Paolo Actis 