Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. We devised a system that uses scanning ion conductance microscopy (SICM) to detect cell surfaces, and voltage pulses to deliver molecules into living cells in culture.

Using double barrel nanopipettes we could independently deliver two different molecules. As a proof of concept, we injected adherent mammalian cells with fluorescent dyes but we are investigating the delivery of biologically relevant molecules (like DNA and proteins).